In HPLC Chromatographic separation Resolution equation is
Rs = 1.18 (tR2 –tR1)/wh1+Wh2,
Where, tR2 and tR1 is run time of eluent ratios of the
distances from the point of application to the centers of
the spots and the distance travelled by the solvent front
from the point of application (retardation factor), and
Wh1and Wh2 , is peak width of at half height.
What is 1.18 ?
Answer Posted / bhavin
In a peak with Gaussian distribution, the peak width is W = 4 σ (where σ is the standard deviation) and the peak FWHM is W0.5h = 2.354σ. hence 1/2*2.354=1.177=1.18
Is This Answer Correct ? | 0 Yes | 0 No |
Post New Answer View All Answers
What is third generation HPLC Columns?
Why we check moisture,ash & AIA in product
Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?
In Assy by potentio metry titration method how select MET U ,DET U, SET U,
why are measure gas flow " ml " in Gas chromatography
IS THERE ANY EQUIPMENT TO CHECK AND CALCULATE THE POLARITY OF A LIQUID?
iam usig ph buffers merk. manually how to prepare ?
CAN WE DETERMINE THE PEAK PURITY IN GAS CHROMATOGRAPHY ANALYSIS?
Which is the highly polar and highly non polar column in HPLC?
Tell me something about Vitamin A test method by HPLC
How do we quantify crystaline and amarpous forms by using (NMR, XRD)spectroscopic techniques? Which any others instruments are useful for this quantification? explain
Why only Copper standard is used to calibrate Atomic Absorption spectrophotometer?
What are usp limits for theoritical plats,resoution,tailing factor,peak to valley ratio
What is the principle of HPLC, GC, LCMS, GCMS,LC QQQ, GC QQQ, LC Q TOF and GC Q TOF. What are the applications and Specificity?
can we use the same detector in HPLC as well GC and what could be the differences we can find in the final chromato graph in any aspects?