MICROBIOLOGICAL ENGINEERING - QUESTION 28.3 : In the calculation of the growth of bacteria, absorbance, A in spectrophotometry is used. According to Beer-Lambert Law, A = e x l x c where A is the absorbance of the solution (no unit), l is the distance of light travels through the solution (in cm), e is the molar absorptivity or the molar extinction coefficient [ in L / (mol.cm) ]. For a particular solute and fixed path length : As / Ao = Cs / Co where Ao is the observed signal for a known concentration Co, and As is the observed signal for a sample concentration Cs. (a) For a cell concentration of 560 cells / mL, a spectrophotometre gives an absorbance reading of 1.0. A mixture of concentration 3600000 cells / mL can be diluted in several operations, with each operation having a dilution of 1:20. How many dilutions should be made so that the concentration of this mixture can be calculated within a range of A = 0.0 to 1.0. (b) In another experiment, a sample tube of 1 cm in width is used. Let A = 0.06 and e = 0.0012 ml / (cell.cm). Find the cell concentration of the sample.
- 1 Answers
- 1711 Views
- I also Faced
- E-Mail Answers
Answer Posted / kangchuentat
MICROBIOLOGICAL ENGINEERING - ANSWER 28.3 : (a) Let Ao = 1.0, Co = 560 cells / mL, Cs = 3600000 cells / mL. By using the equation As / Ao = Cs / Co, then As = Ao x Cs / Co = 1.0 x 3600000 / 560 = 6428.57. For a dilution of 1:20, first stage dilution gives A = 6428.57 / 20 = 321.43, second stage dilution gives A = 321.43 / 20 = 16.07, third stage dilution gives A = 16.07 / 20 = 0.8, which is within a range of A = 0.0 to 1.0. Total stages of dilution needed = 3. (b) By using the equation A = e x l x c, then c = A / (e x l) = 0.06 / { [ 0.0012 ml / (cell.cm) ] x 1 cm } = 50 cells / mL. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
| Is This Answer Correct ? | 0 Yes | 0 No |
Post New Answer View All Answers
Explain how can viscosity affect the design of a mixer?
What are the methods involved to test helium leak testing in a vacuum system?
Explain the different ways in which solids can be blended?
What type of flow measurement devices is best for slurries?
what is the cast iron grade of composition of c-3.06% , Mn- 0.48% , Si-2.38% , S-0.088% , P-0.092% , Cr-0.12% , Ni- 0.012% , Mo-0.00%
At what temperature is glass fused to steel in the making of glass-lined equipment?
I would like to know how to size a partial combustion reactor processing methane and oxygen, as a function of the flowrate and of the pressure.
Why should chemical equation be balanced?
What is some good advice for specifying allowable pressure drops in shell and tube exchangers for heavy hydrocarbons?
What is the maximum permissible Earth to Neutral voltage in an electrical system.
Explain how can you estimate the efficiency of a pump?
Explain how is waste heat boilers categorized?
Should slurry pipes be sloped during horizontal runs?
Is there a difference in mtd (mean temperature difference) between 'e' and 'j' (divided flow) type shell and tube heat exchangers?
Explain about different types of lamps?
