Question 94 - The resolution of separation, Rs for chromatography is given by the formula Rs = (difference in retention time) / (average width at the base). In a chromatogram, 3 peaks a, b and c are found. Average widths W at the bases of the solutes are : Wa = 20 s, Wb = 40 s, Wc = 30 s. Resolutions of separation, Rs for solutes b and c in comparison to a are 2 and 4 respectively. The differences in retention times T for b and c in comparison to a are (Tb - Ta) and (Tc - Ta), Ta = Tc - Tb : (a) Form 2 equations involving Rs as a function of Wa, Wb, Wc, Ta, Tb and Tc. (b) Find the values of Ta, Tb and Tc.
- 1 Answers
- 1827 Views
- I also Faced
- E-Mail Answers
Answer Posted / kangchuentat
Answer 94 - Reference formula Rs = (difference in retention time) / (average width at the base) is used. (a) First equation : Rs = (Tb - Ta) / [ (Wa + Wb) / 2 ] = 2 (Tb - Ta) / (Wa + Wb). Second equation : Rs = (Tc - Ta) / [ (Wa + Wc) / 2 ] = 2 (Tc - Ta) / (Wa + Wc). (b) Substitute Ta = Tc - Tb, Wa = 20 s, Wb = 40 s and Wc = 30 s into first equation and second equation. First equation : Rs = 2 = 2 [ Tb - (Tc - Tb) ] / (20 + 40) = (2 Tb - Tc) / 30, 2 Tb - Tc = 60. Second equation : Rs = 4 = 2 [ Tc - (Tc - Tb) ] / (20 + 30) = Tb / 25, Tb = 100 s. Substitute Tb = 100 s into first equation gives 2 Tb - Tc = 2 x 100 - Tc = 200 - Tc = 60, then Tc = 200 - 60 = 140 s. Then Ta = Tc - Tb = 140 - 100 = 40 s. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
| Is This Answer Correct ? | 0 Yes | 0 No |
Post New Answer View All Answers
Define pneumatic conveying?
Please describe tar, asphalt and bitumine
dear sir/madam, I had applied for the post of officer trainee so plz if u have any papers both technical or aptitude plz inform me on sonalichavan01@gmail.com
Name some guidelines for designing for liquid and gas velocities in process plant piping?
Hi, I am Gurunathan. I am in need of ONGC, IOCL, DRDO technical test ( chemical engineering)and general aptitude questions. If anyone has kindly post it to me soon.
What are the reasons of removal of particles from effluent gas?
hi i need cpcl written exam question papers if anybody pls help me
friends....i have been searching the model paper for HPCL officer trainee for chemical eng....tat apptitude section...if u people got any information regarding tat...please send me to my mail...my id is: maruthu.flower@gmail.com...pls send me wtever information u knew....thank u....
I would like to know how to size a partial combustion reactor processing methane and oxygen, as a function of the flowrate and of the pressure.
Why are decomposition reactions called the opposite of combination reactions?
What types of metals are typically removed via chemical precipitation?
How can wet carbon dioxide be responsible for a corrosion problem in iron-containing metals?
What is the ignition temp. of Aluminum,Coper&Iron.
For amonia manufacturing process if pressure, temperature would be increase over optimum level what would be happen?And what steps should be taken?
How to caliculate the boil up rate, dia and height in both packed and tray distillation columns and any referance books for the design.
