How we develop a method by HPLC for unknown compound,if we got three peaks with same response then how we can find out which one is our desired compound's peak without taking help of LC MS.
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Answer Posted / dinesh solanki
If it is a assay method then inject std in hplc system and compare the chromatogram and identified the interested peak from them.
Same RC method inject std and identified the interested peak and other is may be impurities or contamination of sample.
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