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water is more polar than acetonitrile.but in reverse phase chromatography peaks elute earlier when we increse Acetonitrile.what is the reason.
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Answer Posted / prashant
It based on affinity of eluting substance with stationary phase/mobile phase.if compound elute earlier when we increase ACN obviously compound has more affinity with ACN (towards nonpolar) so it breaks wonderwal bonds with stationary phase and elute earlier
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