Which is the best mobile phase to separate organic acids using C8 column. And how should the samples be prepared for hplc?
Answer Posted / s chatterjee
http://www.restek.com/Technical-Resources/Technical-Library/Foods-Flavors-Fragrances/fff_FFAR2062-UNV
100 % aqueous buffer is the choice, ideally pH 2.8 phosphate
Is This Answer Correct ? | 0 Yes | 0 No |
Post New Answer View All Answers
Why potassium dichromate is used in calibration of uv?
How require to perform linearity as per ANVISA? What are the acceptance criteria?
What is the accrptance criteria in RSD for RS method precision on basis of impurity percentages?
What is importance of pH in the Heavy metal test and How it effect on the test results?
How we performed the force degradation for drug substance, is any specific guideline is available for each parameter(Acidic, basic, oxidation,heat)? what conditions you mentained for above parameters.
Did anybody have method for acetyl cysteine effervescnce tablet
What is the principle of HPLC, GC, LCMS, GCMS,LC QQQ, GC QQQ, LC Q TOF and GC Q TOF. What are the applications and Specificity?
What is split ratio in Gc? Splitless? how requirr to select?
I have compare C2H2-air and C2H2-N2O flame AAS on determination calcium. Both use same range of std to plot calibration curve. (2-6ppm) When i measure the sample with phosphate, KCl and LaCl, C2H2-N2O flame give false positive result, around 0.5ppm. When i measure the sample with phosphste, KCl and EDTA. C2H2-N2O flame also give 0.5ppm false positive. But both above mentioned sample would not give false positive when measured by C2H2-air flame. What is the reason?
how many types of balance calibration test?
Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?
in which situation require to take incident in validation?
why does used dry caffien in HPLC calibration?
pharma industries oriented
Why perchloric acid used for potentiometric titration