What is the principle of GC/HPLC ?
- 4 Answers
- 55808 Views
- BioCan, Biocon, Megafine Pharma, I also Faced
- E-Mail Answers
Answer Posted / manigandan p
This both are separation technique.
GC Principle: Sample vapourised by injection in to a heated system, eluted through column by inert gaseous mobile phase and detect.
HPLC Principle: The solvent liquid will pressurize the sample through column. Column filled with adsorbent material. Each components in the sample interacts slightly differently with adsorbent material. It causes different flow rates for different components and its leading to separate.
| Is This Answer Correct ? | 20 Yes | 0 No |
Post New Answer View All Answers
Why we used in n-butyl acetate water content terminology while in ethyl acetate we used moisture content terminology?
why cone formation during dissolution?
Difference between hlaf and rlaf
effect of pore size, pore volume, partical size, column length, carbon load on retention time? what is carbon load? what is the use?
What are usp limits for theoritical plats,resoution,tailing factor,peak to valley ratio
What is the difference between Discriminating media and DPDM(Dissolution Profile with Different Media)
if your impurity coeluting with each other in that situation how require to set specification? is it acceptable?
What is similarity factor and its use in api strength?
why require to do water content for drug product?
what is third integration
which situation gc hs and gc als require to use?
WHY given much noise PDA dector then VWD Dector
How to determine the EDTA content by potentiometry titration in Ceftriaxine sodium
What is viod volume and peak purity in HPLC?
we are performed SOR for a particular product the limit is -6 to -10.We face particular bathes it is not meeting the specification.we performed diffrent instrument diffrent analyst we are getting diffrent diffrent results.solvent is dimethyl sulfoxide what could be the reasion
