What is the principle of GC/HPLC ?
Answer Posted / manigandan p
This both are separation technique.
GC Principle: Sample vapourised by injection in to a heated system, eluted through column by inert gaseous mobile phase and detect.
HPLC Principle: The solvent liquid will pressurize the sample through column. Column filled with adsorbent material. Each components in the sample interacts slightly differently with adsorbent material. It causes different flow rates for different components and its leading to separate.
| Is This Answer Correct ? | 20 Yes | 0 No |
Post New Answer View All Answers
how require to fix the linearity and accuracy cincentration range?
How to establish relative response factor for hplc. Why it is required?
What is delay volume?
What is the formula to determine the concentration of M of a solution given the % transmittance? Use %T = 43.7 as an example.
what is mean by nitrosamine impurities?
Why only Copper standard is used to calibrate Atomic Absorption spectrophotometer?
Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...
If we have 5 strength which is not dose proportinate and excipients also diffrent in each strength then how we can proceed for Force degradation? and excipient are same but not dose propotinate the how FD?
on the basis of bcs class how to proceed the solubility?
If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately
function of detecter in hplc ,gc and spectroscopy? function of carrier gas in gc?
If inhouse hplc related substance method is completly diffrent from Usp for finished proďuct with diffrent impurities then how require to prove method equivalecy?
why sre you used Potassium hydrogen phthalate in standarisation of 1N NaOH and 0.1 N Perchloric Acid?
What is related substance by HPLC impurity limits as per USP?
in which situation require to take incident in validation?