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in hplc calibration we are using 0.05% acetone why we are
using that one and what happen when its conc increases or
decreases
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Answer Posted / a.iyyappan
0.05% Acetone is used in HPLC GPV(Gradient performance verification)test, since its heigher level of absorption.
Not only Acetone, caffeine also recommended for this test.
0.05% Acetone and Caffeine are recommended by HPLC manufacture(Shimadzu).
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