why we inject sample TWICE in ASSAY by HPLC analysis but in RS once?

Answer Posted / abdul khadar shaik

Related compounds:Generally,We are using two preparation and two injections by HPLC and GC.After compleation of Related compounds validation,we are using one preparation and one injection.

Is This Answer Correct ?    0 Yes 5 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

From where require to take the RLD sample?

282


As per ICH related substances stability trend limit from initial to shelf life

2668


Colorimtry

1174


1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer

1304


we can use expired sample for validation and analyst qualification?

301






iam usig ph buffers merk. manually how to prepare ?

1781


in hplc chromatogram started from left to right and in uv spectrum started from left to right

471


what is the importance of peak purity in HPLC and how we can calculate through manul(not software)?

3988


If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately

359


identification is for unknown? qualification for known? reporting for LOQ?

286


how you determine moist.of NaOH sol or NaOH flacks

1770


effect of pore size, pore volume, partical size, column length, carbon load on retention time? what is carbon load? what is the use?

328


if peak get problamatic then what require to do?

319


How require to develop GC method? how to select diluent, GAS, column selection and other chromatographic conditions?

277


How to determine water content of bis tetrazole amine mono ammonium salt

2966