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Difference between c18
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Answer Posted / jeevithan
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18 & C8 reverse-phase column (particle size 3 & 5 µm) can be used to separate low molecular weight protein and peptides. Anyone can suggest me the "specification of particle size" of HPLC column for high MW protein?
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? - ResearchGate. Available from: https://www.researchgate.net/post/Which_type_of_column_should_i_use_to_check_the_purity_of_high_molecular_weight_protein_using_HPLC_reverse_phase_column_chromatography [accessed Jun 27, 2015].
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