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what is the difference between potentiometric assay and
hplc assay
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Answer Posted / viswanathan
HPLC:
Exact quantification method.in case of any impurity present in the same it will eluted in the different RT.
Potentiometer:This also quantification method,it will not suitable for all the sample.Because if any impurity present in the sample it will consume the titrant.so that it is not correct method.
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2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
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