Answer Posted / manoj k kasar

It is not compulsary to use acetone, if we use any compound(which is soluble in particular solvent) having maximum absorbsnce at particular wavength,then also we got the stepwise calibration like acetone gradiant stepwise calibration in hplc.

If ur time program is correct to obtain stepwise calibration of acetone then nothing will happens if u increase or decrease the conc. of acetone.

what is difference between UV - VISIBLE MODEL NO like 1600,1601,1700 etc ? plz explain me

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what is definition of validation? which components are followed give detail?

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[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?

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how you start the solubility study?

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before starting analytical method valodation what you checking? and how giving preference to start validation?

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