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In HPLC when we use Isocratic method, Gradient method in
hplc?
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Answer Posted / ramesh artemis biotech hyd
A separation in which the mobile phase composition remains constant throughout the procedure is termed isocratic (meaning constant composition). the mobile phase composition does't have to remaian constant. A separation in which the mobile phase composition is changed during the separation process is described as a gradient elution. Then isocratic method used fro constant composition position of mobile phase and gradent method used for mobile phase is low to high concentration.
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