In HPLC when we use Isocratic method, Gradient method in
hplc?
Answer Posted / ramesh artemis biotech hyd
A separation in which the mobile phase composition remains constant throughout the procedure is termed isocratic (meaning constant composition). the mobile phase composition does't have to remaian constant. A separation in which the mobile phase composition is changed during the separation process is described as a gradient elution. Then isocratic method used fro constant composition position of mobile phase and gradent method used for mobile phase is low to high concentration.
| Is This Answer Correct ? | 3 Yes | 4 No |
Post New Answer View All Answers
what are risk assement in the analytical qbd?
Why Ethanol is Used for Standardization of GC Head space?
Why use only methanol,used determinationof moisture content not use other solvents
WHAT IS THE USE OF STARTER ?????????
Colorimtry
what you do when compound having dwel wavelength?
why should we perform dissolution PVT calibration only by UV spectrometer, not by HPLC ?
if rsd failed then what require to do?
How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.
how to set sample and standard concentration in RS method?
what is the extinction coefficient for Indomethacin or at 319nm wave length.
what is mean by nitrosamine impurities?
how to selecet an exact coloumn for an new molecule development by hplc how to select exact salt as buffer for new molecule development by hplc what is the the process to select the mode of saparation of compoundes by hplc what is the use of ph of buffer what is use of buffer,ph,organic phase,ans methods how the molecules get saparated in coloumn,
if identification threshold crosses the limits then what next step?
from where i get mortar pastle for glass bottle crush? it's required for testing of it?