Is there any difference in principal of reverse phase and
normal phase chromatography? and why?
Please update.
Thank and regard
Answer Posted / kishore
Chromatography consists single principle. that is
separation technique. in the chromatography two phases are
there.
1) Reverse phase(separation with polar mobile phase)
2) Normal phase(separation with non polar mobile phase)
there is no difference in the principle of mechanism of
chromatography. only difference in the phase, what we
selected for the separation of components.
| Is This Answer Correct ? | 29 Yes | 2 No |
Post New Answer View All Answers
Difference between hlaf and rlaf
What is Band broading effect explain
if you have given a blank water how you analyse by general analytical techniques?
Related substance method equivalency on control sample or spiked sample?
effect of pore size, pore volume, partical size, column length, carbon load on retention time? what is carbon load? what is the use?
How you develop a method in HPLC?
why does used dry caffien in HPLC calibration?
why octanol used to determine the partition coefficient ?
WHY given much noise PDA dector then VWD Dector
If vendor having more imp than monograph then how to proceed? and how to set spec?
what you do when compound having dwel wavelength?
what is the principle involved in turbidometry and nephelometry ?
If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately
Ph meter can show more than 14 ph reading? Why ph range in between 1 to 14 only?
in which situation ion pair require to use?