In HPLC when we use Isocratic method, Gradient method in
hplc?
- 8 Answers
- 19919 Views
- Pharmaceutical, I also Faced
- E-Mail Answers
Answer Posted / keerthi achanta
for method development, better to use gradient method
because we are try to develop the method by changing the
different composition of mobile phase.
once if we know the method then we can use isocretic method
| Is This Answer Correct ? | 21 Yes | 10 No |
Post New Answer View All Answers
How do we fix the sample concentaryion in hplc method development. What is the basis?
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18
how you start RS method development when for new product?
Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...
WHY given much noise PDA dector then VWD Dector
We use Potassium Dichromate solution for the calibration of UV-Visible Spectrophotometer in UV region. My question is to calibrate visible region which solution could be used in photometric accuracy
if instrument calibration fails then what require to do?
My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards
what is different when impergnated silica plates are used in separation of azo dyes using column chromatography?
For standardization of volumetric solutions, acceptance limit for concentration is considered as 10% of nominal concentration and %RSD as NMT 0.2% (for appropriate no. of replicates). But , if %RSD has not met, what should be further action?
how can I make the copper get white bye any salt or acit?
how to calculate elemental impurities?
In which situation we require to analytical method validation of excipient?
what is mean by extactable and leachable study?
