wen v have polr solvent then which type of column use in hplc

Answer Posted / raj

Non-polar columns are used( eg:C18 )

Is This Answer Correct ?    15 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

why xterra column require to use at higher ph?

436


How can the GC or HPLC method is selected to determine the impurity profile in drug product?

6869


sop of a uv visible spectrophotometer double beam elico model

5358


How can we calculate offset value pH measurment

1075


What is the diffrence in japan mkt requirement in analytical method validation over US?

301






can i use hplc detector to uplc and why?

1817


How to compare XRD graphs against standard and carry polymorpism study of API's by powder XRD method?

3396


WHICH STANDARD WE USE IN SILICA ANALYSIS IN SILICA-GEL METHOD FOR SOLID SAMPLE

1621


In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?

1462


WHY pKa value is more important than pH value of the mobile phase in HPLC?

1262


acceptance criteria for lod & loq by standard deviation of response and slope??

2098


why octanol used to determine the partition coefficient ?

1922


Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18

1462


In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.

2614


How you develop a method in HPLC?

2083