water is more polar than acetonitrile.but in reverse phase chromatography peaks elute earlier when we increse Acetonitrile.what is the reason.

Answer Posted / nisha

water is more polar then acetonitrile so it make bond strongly than acetonitrile with stationary phase so its viscocity is lowered

Is This Answer Correct ?    4 Yes 4 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

How do we get end points and how many end points are possible for citric acid and di-acid not theorotically answer should be given practically.

2055


How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?

2871


what is lod and loq ?,why use k2cr2o7 , kcl h2so4 in uv calibration ?,why use benzophenone & caffene acetone in hplc calibration ?,what is leading peak in hplc ?why we do the calibration of limit of stry light in hplc & uv ?

3193


How will u perform degradation study in detectors other thann PDA in HPLC Methodology

2023


What is the difference between spectro meter and spectro photo meter?

10433






what is the acceptance criteria for enteric coated tablets in 0.1n hcl validation in each parameter?

349


HI,I CLEARED BOB CLERK EXAM. MY INTERVIEW WILL BE ON 9TH OCTOBER,2010.PLEASE SEND ME INTERVIEW QUESTIONS AND ANSWERS. THANK YOU.

1739


how you start the solubility study?

346


2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.

1768


i want model written test paper for the post of analyst in IOCL(indian oil corporation ltd) any one pls help me

2189


How would you decide dissolution medium for NCE compound of class I drug

1711


how you determine moist.of NaOH sol or NaOH flacks

1772


Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?

2478


How can the GC or HPLC method is selected to determine the impurity profile in drug product?

6869


Ph meter can show more than 14 ph reading? Why ph range in between 1 to 14 only?

308