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Answer Posted / azhar ali
Principle of gas
chromatography:
The sample
solution
injected into
the instrument
enters a gas
stream which
transports the
sample into a
separation tube
known as the
"column." (Helium
or nitrogen is
used as the so-
called carrier
gas.) The
various
components are
separated inside
the column. The
detector
measures the
quantity of the
components that
exit the column.
To measure a
sample with an
unknown
concentration, a
standard sample
with known
concentration is
injected into
the instrument.
The standard
sample peak
retention time
(appearance
time) and area
are compared to
the test sample
to calculate the
concentration.
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