water is more polar than acetonitrile.but in reverse phase chromatography peaks elute earlier when we increse Acetonitrile.what is the reason.
- 10 Answers
- 49015 Views
- Matrix, I also Faced
- E-Mail Answers
Answer Posted / ronda.venki
based on polarity of stationary phase and sample, normally
reverse phase columns having stationary phase non polar so
ACN INCREASE means elution strength increased bcz of
interactions of stationary phase and product
| Is This Answer Correct ? | 16 Yes | 2 No |
Post New Answer View All Answers
AT WHAT CONDITIONS WILL YOU ANALYSE A SAMPLE,WHICH ARE PRESERVED AT OTHER THAN THE AMBIENT CONDITIONS?(ie COLD STORAGE SAMPLES BELOW 20* CENTIGRADE?
Colorimtry
In which situation we require to analytical method validation of excipient?
why does used dry caffien in HPLC calibration?
Why use only methanol,used determinationof moisture content not use other solvents
why xterra column require to use at higher ph?
analytical method validation require to with respect to release specification or shelf life specification?
How doing qbd practically?
WHAT IS THE CRITERIA FOR SELECTION OF TIME OF DISSOLUTION AND THE MEDIUM OF DISSOLUTION?
WHICH STANDARD WE USE IN SILICA ANALYSIS IN SILICA-GEL METHOD FOR SOLID SAMPLE
Why we check moisture,ash & AIA in product
how to selecet an exact coloumn for an new molecule development by hplc how to select exact salt as buffer for new molecule development by hplc what is the the process to select the mode of saparation of compoundes by hplc what is the use of ph of buffer what is use of buffer,ph,organic phase,ans methods how the molecules get saparated in coloumn,
what is the difference between potentiometric titration and karl fischer titration?
Identify problem faced when mass spectroscopy is used with HPLC system ?
1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer
