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what is the relative response factor in related substances?
is it necessary to calculate?
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Answer Posted / krishna vakharia
A measure of the relative response of the instrument
detector to an analyte compared to an internal or external
standard. Relative Response Factors are determined by the
analysis of standards and are used to calculate the
concentrations of analytes in samples. The RRF can be
calculated using the following equation:
RRF = (Ac x Cis)/(Ais x Cc)
where,
RRF = Relative response factor
Ac = Area of the target analyte
Ais = Area of the corresponding internal standard
Cis = Concentration of the corresponding internal standard
Cc = Concentration of the target analyte
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