Interested to Buy Any Domain ? << Click Here >> for more details...
Answer Posted / noreen
HPLCis a separation techniquethat involves:
•the injection of a small volume of liquid sample
•into a tube packed with tiny particles (3 to 5 micron (μm)
in diameter called the stationary phase)
•where individual components of the sample are moved down
the packed tube (column) with a liquid (mobile phase)
forced through the column by high pressure delivered by a
pump.
In principle, LC and HPLC work the same way except the
speed,efficiency, sensitivity and ease of operation of HPLC
is vastly superior.
•These components are separatedfrom one another by the
column packing that involves various chemical and/or
physical interactions between their molecules and the
packing particles.
•These separated components are detected at the exit of
this tube (column) by a flow-through device (detector) that
measures their amount. An output from this detector is
called a “liquid chromatogram”.
| Is This Answer Correct ? | 1 Yes | 0 No |
Post New Answer View All Answers
seminal on pyrimidine
Why do we use vaccum in Loss on drying test?
Actinium is a radioactive element. How is its radioactivity utilized in various practical applications or fields?
What is a repartition coefficient?
why use of sulfuric acid in roi test in pharmaceutical industry
Protactinium-233 is used in the thorium fuel cycle. Explain how this cycle operates and its potential benefits in nuclear energy production.
What is the difference between cracking, pyrolysis, destructive distillation, hydroforming, carbonization and dry distillation?
Protactinium is a rare and highly radioactive element. How is its radioactivity utilized in various scientific and industrial applications?
can you pls provide chemistry previous papers for the post of trainee chemist in ap genco
how to prepare for the exam of ongc please give some idea
As a chemist working with Actinium, what safety precautions and protocols would you follow due to its radioactive nature?
Protactinium has a relatively short half-life for some of its isotopes. How does this characteristic impact its practical uses and research?
Actinium is part of the actinide series. Could you explain the significance of this series and its placement in the periodic table?
Barc in chemistry
why we not use caffeine at 205nm for hplc calibration?
