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Answer Posted / noreen
HPLCis a separation techniquethat involves:
•the injection of a small volume of liquid sample
•into a tube packed with tiny particles (3 to 5 micron (μm)
in diameter called the stationary phase)
•where individual components of the sample are moved down
the packed tube (column) with a liquid (mobile phase)
forced through the column by high pressure delivered by a
pump.
In principle, LC and HPLC work the same way except the
speed,efficiency, sensitivity and ease of operation of HPLC
is vastly superior.
•These components are separatedfrom one another by the
column packing that involves various chemical and/or
physical interactions between their molecules and the
packing particles.
•These separated components are detected at the exit of
this tube (column) by a flow-through device (detector) that
measures their amount. An output from this detector is
called a “liquid chromatogram”.
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