Answer Posted / nimi narayanan

The major difference between gram positive and gram
negative bacteria lie in the chemical compostion of their
cell wall.
1. Gram +ve bacteria has got higher amount of peptidoglycan
compared to gram negatives. this cross linked peptidoglycan
is responsible for retaining the crystal violet during gram
staining.
2. Gram -ves has lower peptidiglycan but has got a lipid
layer in the cell wall. tHis lipid is gets extrcted away
during alcohol destaining in gram's stainig proceduremaking
the cell wall more permeable and thus crystal violet is not
reained and take the counterstain saffranin
3. gram+ves has teichoic acid in cell wall but gram -ves do
no not.
4.gram -ve cerll wall has got lpopolysaccharides which is
responsible for its endotoxic and antigenic property

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