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If you have given an unknown compound how you develope a
hplc analysis method for anlysis?
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Answer Posted / dheeraj pandey
FIRST WE SELECT THE SOLUBILITY OF THE UNKNOWN SAMPLE AND
MAKE THE DILUTION AND OBSERVE THE ABSORBANCE IN UV AT
SPECTRUM MODE AND SELECT THE LAMDA MAX AND ON PHOTOMETRIC
MODE OBSERVE THE CHRMATOGRAM AFTER THAT WE SELECT A MOBILE
PHASE ON THE SOLUBILITY OF SAMPLE AND DEVELOPED THE METHOD
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