water is more polar than acetonitrile.but in reverse phase chromatography peaks elute earlier when we increse Acetonitrile.what is the reason.
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Answer Posted / paresh jobanputtra, piramal(ah
Actually What happen,When we mix Acetonitrile with buffer then VISCOSITY is decresed of mobile phase.
"As viscosity decreased, Mass transfer of solute particle with stationary phase is decreased"
So but obvious component elute earlier.
Regards,
Paresh Jobanputtra
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