Interested to Buy Any Domain ? << Click Here >> for more details...
Answer Posted / visweswara rao
Gram's staining is the one used for staining the bacteria
to determine whether the bacteria is Gram positive or Gram
negative.
the process of Gram staining is
1) Take a loopful of bacterial culture and place it on
the clean and sterilised slide.
1) Fix smear by heat.
2) apply crystal violet.
3) Wash with water
4) Cover with Gram’s iodine
5) Wash with water. do not blot.
6) Decolourize for 10-30 sec with gentle agitation in
acetone(30ml)and alcohol(70ml).
7) Wash with water. do not blot.
8) Cover for 10-30 sec with safranin(2.5% solution in
95% alcohol.)
9) Wash with water and let dry.
The bacteria depend on the colour absorbtion by the cell
wall is classified into two types. They are 1.Gram psoitive
bacteria (purple) 2. Gram negative bacteria(red or pink
colour)
| Is This Answer Correct ? | 15 Yes | 1 No |
Post New Answer View All Answers
What is the definition of 'biomedical'?
how do you get on with your current boss
what is biological hydrogen production? 0 7 Hai friends, Can someone guide me..Am a postgraduate(m.tech) in biotechnology and am thinking of doing pg diploma course through distance mode. are they worth doing? if so which is better pg diploma in bioinfo or pg diploma in Intellectual property rights..am awaiting ur suggestions 0 20 hey i am doin bsc biotech and i want to do research on stem cells.....plz tell me how to go about?????? 0 32 in the lcmv what type of effect onm the mhc class i molecule 0 18 WHAT IS MEAN BY GENETIC ALGORITHM 1 56 Can I get a working model on petro plants ? 0 76 if i m doing bsc in biotechnology now.. are there any chances that i can do b.tech nw?? 0 59 what is multicolour biotechnology? what are the applications of multicolour biotechnology? 4 275 will i get f1 visa to pursue masters in US, if i have many backlogs and more than 2 yrs gap in btn my graduation. 0 85 suppose if u have gone through any biotech industry, what are the environmental conditions that u r expecting in quality control? Shanta-Biotech 2 598 suppose if u have gone through any biotech industry, what are the environmental conditions that u r expecting? Shanta-Biotech 1 261 what is the process of chees preparation 1 240 how biodiesel helps in reducing co2 1 313 what is multi colour bio technology? 1 200 i m doing bsc in biotechnology n i m confuse what to do in future...whether i shall do msc in biotechnology or any otyer course?in future which field will have greater oppurtunity?
what are the shine-dalgarno sequences in organism other than E.coli.?
How interferon is produced?
in the lcmv what type of effect onm the mhc class i molecule
SIR I AM APPEAR M.Sc PREV(BIOTECHNOLOGY) I KNOW BIOTECNOLOGY SCOPE IN INDIA
Can I get a working model on petro plants ?
Explain about cytokines?
Sir i am doing my btech biotech at dy patil pune nd due to some prob i need to shift backbto surat so is it possible to transfer at bsc biotech college in surat
on burning normaly wich parts of our body geting less dameg? why?
Why do you use dbm instead of ncf in the northern blotting and what are the pre-treatments required for this paper to be used?
could u please say me the job opportunities for M.Sc biotech students in India
I completed Masters in Biotechnology in 2003.I have around 5 years experience in Microbiology.But iam interesterd in doing Biotechnology work.Please help how to get job in biotech field?
I am doing my m.sc biotechnology.but i don't know about the interview questions of various company and how i have to face.can you please anyone send that placement papers?thank you
