Interested to Buy Any Domain ? << Click Here >> for more details...
what is the difference between c-8 and c-18 column
- 7 Answers
- 24496 Views
- I also Faced
- E-Mail Answers
Answer Posted / bhupalreddy
In chromatography silanol groups (Si-OH) at the
surface of silica gel are reacted with substituted Chloro
Silanes.
CH3 CH3
| | | |
--Si–OH + Cl - Si--R → --Si-O - Si - R
| | | |
CH3 CH3
By changing characteristics of group i.e. from changing R
from C1 to C18 we can alter the nature of stationary phase
from polar to non polar. Since C18 columns is non polar.
This helps separating various combinations of polar – non
polar constituents from the analyte.
C-8 columns contain 8 carbons and C-18 contains 18
carbons are attached to silicon group to form stationary
phases for chromatography. The C-18 columns are highly
Non polar compared to C-8 columns and most of the compounds
are well separated in C-18 carbon packed columns.
| Is This Answer Correct ? | 14 Yes | 2 No |
Post New Answer View All Answers
what is classification of elemental impurities? what is risk assement in elemental impurities?
[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?
What is the difference between Discriminating media and DPDM(Dissolution Profile with Different Media)
how can give the expiry period and restadardisation of volumetric solution
in DMF having extra impurities and in api COA also having extra imp than USP or BP product then how require to proceed?
What are modes of quantification in related substances
WHAT IS THE PURPOSE WE USE HCL, CHOPPER, BURNER, MONOCHROMATOR in AAS? PLEASE EXPLAIN BRIEFLY
Loss of linearity in tkn analysis, possible reasons?
How to choose the which salt is suitable for mobilephase
We use hicrome 10 cxs column in hplc analysis....pls tell me.. what is the meaning of 10 cxs?
What is column in chromatography?
WHY given much noise PDA dector then VWD Dector
how to calculate elemental impurities?
What is importance of pH in the Heavy metal test and How it effect on the test results?
If inhouse hplc related substance method is completly diffrent from Usp for finished proďuct with diffrent impurities then how require to prove method equivalecy?
