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Answer Posted / rishu
Enables proteins and peptides to be sequenced.
Pehr Edman introduced phenylisothiocyanate (Edman's
reagent) to selectively label the amino-terminal residue of
a peptide. In contrast to Sanger's reagent, the
phenylthiohydantoin (PTH) derivative can be removed under
mild conditions to generate a new amino terminal residue
(Figure 4–7). Successive rounds of derivatization with
Edman's reagent can therefore be used to sequence many
residues of a single sample of peptide. While the first 20–
30 residues of a peptide can readily be determined by the
Edman method, most polypeptides contain several hundred
amino acids. Consequently, most polypeptides must first be
cleaved into smaller peptides prior to Edman sequencing.
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