Answer Posted / bhagavanji nelakanti
Masking of secondary silanol groups of bonding phase by tri
methyl silane (TMS)molecules is called as end capping. In
the bonded phase the secondary silanol molecules which shows
high polar bonding property with compound retains in the
column and those compounds elute slowly. Because of the end
capping the binding property of the secondary silanol
molecules will reduce and resulting less retention of those
compounds.
| Is This Answer Correct ? | 45 Yes | 3 No |
Post New Answer View All Answers
What is the difference between Discriminating media and DPDM(Dissolution Profile with Different Media)
which situation gc hs and gc als require to use?
If change in specification which parameter require to do for validation? if change in chromtographic condition then which parameter? if api change then which parameter? if change composition then which parameter? if old method not work out then whicj parameter? if additional one impurity added then which parameter of validation require to do on above each conditions? elaborate separately
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18
Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?
Why a1% value is used for some product ? What is the criteria for selection a1% value ?
USP methodology, EP methodology, IP methodology, among three if possible to use one methodology for qualify working standard to use USP, EP, IP ? Please explain...
WHY given much noise PDA dector then VWD Dector
What is delay volume?
How to compare XRD graphs against standard and carry polymorpism study of API's by powder XRD method?
Explain the relations between number of carbon atoms in alkanes and retention time ?
How can the GC or HPLC method is selected to determine the impurity profile in drug product?
for heavy metal test lead used in which form pure form or any other form for preparation of lead standard sol
How to determine water content of bis tetrazole amine mono ammonium salt
What is the diffrence in japan mkt requirement in analytical method validation over US?
