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How to calculate LOD & LOQ in HPLC Validation Method

Answer Posted / sunil.madhukar rao kulkarni

We can calculate LOD and LOQ values using linearity
graph,insted of sigma value STEYX can be considered for
more acuurate vale of LOD and LOQ

where STEYX : Standard error of the pridected Y-value for
each X in a regression

Final calulation given as below :

LOD = STEYX x 3/ Slope

LOQ = STEYX x 10 /Slope

Is This Answer Correct ?    44 Yes 10 No



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