How to calculate LOD & LOQ in HPLC Validation Method
Answer Posted / sunil.madhukar rao kulkarni
We can calculate LOD and LOQ values using linearity
graph,insted of sigma value STEYX can be considered for
more acuurate vale of LOD and LOQ
where STEYX : Standard error of the pridected Y-value for
each X in a regression
Final calulation given as below :
LOD = STEYX x 3/ Slope
LOQ = STEYX x 10 /Slope
| Is This Answer Correct ? | 44 Yes | 10 No |
Post New Answer View All Answers
WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?
What are usp limits for theoritical plats,resoution,tailing factor,peak to valley ratio
what is procedure of actual procedure for registration of drug?
why cone formation during dissolution?
We use Potassium Dichromate solution for the calibration of UV-Visible Spectrophotometer in UV region. My question is to calibrate visible region which solution could be used in photometric accuracy
what is mean by dissolution hydrodynamics?
why we should take dst factor for below 1%moisture samples
if you get peak in blank then what require to do?
USP methodology, EP methodology, IP methodology, among three if possible to use one methodology for qualify working standard to use USP, EP, IP ? Please explain...
why octanol used to determine the partition coefficient ?
From where require to take the RLD sample?
What is intact assay method development.....?
in which situation require to use paddle and basket?
in DMF having extra impurities and in api COA also having extra imp than USP or BP product then how require to proceed?
can i use hplc detector to uplc and why?