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Why caffeine as a reference standard used for calibration
of HPLC?
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Answer Posted / rohidas baban shinde
Because it shows maxima at the wavelength of 205 nm and 273
nm and shows and also shows minima at the wavelength of 245
nm. It is a Primary reference standard and it is avaliable
in the market very easily.It is cheap.It is non-degradable
and we can used it for longer period of analysis.
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