Interested to Buy Any Domain ? << Click Here >> for more details...
Answer Posted / swapna
The northern blot is a technique used in molecular biology
to study gene expression. It is similar to the Southern blot
procedure, difference is that RNA, rather than DNA, is the
substance being analyzed by electrophoresis and detection
with a hybridization probe.
Main difference with Southern blot is the addition of
formaldehyde in the agarose gel, which acts as a denaturant.
As in the Southern blot, the hybridization probe may be made
from DNA or RNA.
reverse northern blot. In this procedure, the substrate
nucleic acid is a collection of isolated DNA fragments, and
the probe is RNA extracted from a tissue and radioactively
labelled.
| Is This Answer Correct ? | 7 Yes | 5 No |
Post New Answer View All Answers
What are the conditions of sterilization in an autoclave?
Give the full scientific name (genus and species) name for a xylem-limited fastidious bacterium?
Why Lactobacilli are considered the most aciduric and acidogenic organisms in the oral cavity ?
Explain the Recipe for MM1 media ?
Which are the various dilutions of sulfuric acid used?
What are conditions under which a direct flame may be used to effect sterilization?
what is the method for seperation of periplasmic proteins ?
Name a fungus that is gram positive?
Explain the meaning of bioremediation and describe four examples where microorganisms have been used or can be used in bioremediation?
What do you know about Microbial growth database ?
Something about pbr322 and cloning blunt ended insulin ?
Why does frame shift mutation (GC gase paired) occurs due to the pesticides in TA98 salmonella tester strain? What is its reason and the mode of action of the pesticides on the CG sequence of the DNA of TA98 strain
How to stock culture of anaerobic bacteria?
What are the Problems associated with P1 transduction?
Describe the antiviral activity screening facilities ?
