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Answer Posted / swapna
The northern blot is a technique used in molecular biology
to study gene expression. It is similar to the Southern blot
procedure, difference is that RNA, rather than DNA, is the
substance being analyzed by electrophoresis and detection
with a hybridization probe.
Main difference with Southern blot is the addition of
formaldehyde in the agarose gel, which acts as a denaturant.
As in the Southern blot, the hybridization probe may be made
from DNA or RNA.
reverse northern blot. In this procedure, the substrate
nucleic acid is a collection of isolated DNA fragments, and
the probe is RNA extracted from a tissue and radioactively
labelled.
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