some compound havving uv region but not give much more
response during hplc analysis
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Answer Posted / ashvin
During Hplc analysis UV nm lamda not proper regards to the
compound or having wrong mobile phase so eluent not properly
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2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
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