how peak can be sharp in hplc when it is come flat
Answer Posted / vivek k
Sample solvent stronger than the mobile phase Dissolve
sample in mobile phase or at least reduce the strength of
the sample solvent as much as possible
Sample mass overload Reduce the amount (mass) of sample
injected.
Silanol interaction with amines • Reduce mobile phase pH to
< 3.0
• Increase mobile phase ionic strength.
25mM to 50mM recommended
• Add a competing amine to the mobile phase.
10 mM TEA is usually sufficient.
• Select a stationary phase with a lower silanol activity.
Adsorption of acids on silica • Increase salt
concentration in the mobile phase
25 mM to 50 mM is usually sufficient
• Reduce the pH of the mobile phase to < 3.0
• Add a competing organic acid.
1% acetic acid or 0.1% TFA is usually sufficient
Column void Replace the HPLC column.
Attempts to fill-in the void are seldom worth the effort.
Is This Answer Correct ? | 10 Yes | 0 No |
Post New Answer View All Answers
How to establish relative response factor for hplc. Why it is required?
Why sodium hydroxide used for maintain pH of phosphate buffer
WHICH STANDARD WE USE IN SILICA ANALYSIS IN SILICA-GEL METHOD FOR SOLID SAMPLE
function of detecter in hplc ,gc and spectroscopy? function of carrier gas in gc?
we can use expired sample for validation and analyst qualification?
what are risk assement in the analytical qbd?
why sre you used Potassium hydrogen phthalate in standarisation of 1N NaOH and 0.1 N Perchloric Acid?
can we use the same detector in HPLC as well GC and what could be the differences we can find in the final chromato graph in any aspects?
Which products will produce when acetic anhydride reacts with Ter-butanol, Sec-butanol, Iso butanol, Dimethylamine etc.
what is biorelivent dissolution media?
In Assy by potentio metry titration method how select MET U ,DET U, SET U,
mahagenco syllabus for the post of chemist
how you fix the limits of impurities?
what is the difference between potentiometric titration and karl fischer titration?
what is the purge flow & how to calculate