Interested to Buy Any Domain ? << Click Here >> for more details...
what is the difference between gc& hplc. why we r not
calculating purity by gc for final products
- 5 Answers
- 17528 Views
- Biocon, Cipla, I also Faced
- E-Mail Answers
Answer Posted / dhanalakshmi
Gas chromatography (GC) is based on a partition equilibrium
of analyte between a solid stationary phase (often a liquid
silicone-based material) and a mobile gas (most often Helium).
In the HPLC technique, the sample is forced through a column
that is packed with a stationary phase composed of
irregularly or spherically shaped particles, a porous
monolithic layer, or a porous membrane by a liquid (mobile
phase) at high pressure
GC is used for volatile compounds (those that break down
rapidly) while HPLC is better for less volatile samples. If
a sample contains salts or carries a charge, it must be
analyzed using HPLC, not GC
GC is used for volatile compounds (those that break down
rapidly) while HPLC is better for less volatile samples. If
a sample contains salts or carries a charge, it must be
analyzed using HPLC, not GC
| Is This Answer Correct ? | 25 Yes | 3 No |
Post New Answer View All Answers
How to determine water content of bis tetrazole amine mono ammonium salt
iam usig ph buffers merk. manually how to prepare ?
EXPLAIN THE CALCULATION PART OF AN KF TITRATOR CALIBRATION WITH SUITABLE EXAMPLE
How to choose the which salt is suitable for mobilephase
mode of absorption in alimentary canal?
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18
what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?
In the HPLC Calibration done wavelength accuracy done between 200nm-280nm .but not done remaining 300-400nm not done ?
why we are using benzene, anyline in acetic anhdride assay titration method?
how you fix the limits of impurities?
how you start RS method development when for new product?
how can I make the copper get white bye any salt or acit?
Why we use potassium dichromate in uv calibration Exact reason behind it??
How to calculation of residual solvent methanol (3000 ppm) standard solution, and how many quantity used of test sample
