what is the difference between gc& hplc. why we r not
calculating purity by gc for final products
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Answer Posted / dhanalakshmi
Gas chromatography (GC) is based on a partition equilibrium
of analyte between a solid stationary phase (often a liquid
silicone-based material) and a mobile gas (most often Helium).
In the HPLC technique, the sample is forced through a column
that is packed with a stationary phase composed of
irregularly or spherically shaped particles, a porous
monolithic layer, or a porous membrane by a liquid (mobile
phase) at high pressure
GC is used for volatile compounds (those that break down
rapidly) while HPLC is better for less volatile samples. If
a sample contains salts or carries a charge, it must be
analyzed using HPLC, not GC
GC is used for volatile compounds (those that break down
rapidly) while HPLC is better for less volatile samples. If
a sample contains salts or carries a charge, it must be
analyzed using HPLC, not GC
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