Answer Posted / balaji

There are three different method indiated in ICH guidelines
for determination of LOD and LOQ.

1 Visual evaution method - For non instrument and sometimes
for instrumental also.

2 Slope Method:Based on residual standard deviation of the
response and
the slope
obtained will be calculated as:

Limit of Detection (LOD):
DL= 3(RESIDUAL SD/Slope)


Limit of quantification(LOQ):
DL=10(RESIDUAL SD/Slope)

3 Signal to noise ratio method
Limit of Detection (LOD):
S/N NLT 3

Limit of quantification(LOQ):
S/N NLT 10
Slope and S/N methods are more reliable
method for determination of LOD and LOQ value

In rs method development when we are going area normalization method to dilute standard method?

1486

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2278

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How will u perform degradation study in detectors other thann PDA in HPLC Methodology

2496

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How do we fix the sample concentaryion in hplc method development. What is the basis?

1160

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how you establish the LOQ?

738

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what is mean by peak intigrity?

886

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how to qualify the impurity?

805

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why we use a particular hplc column for a particular compound give reasons?please

2703

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inhouse product is in capsule form in combination and RLD is in tablet form then can we proceed for multimedia CDP? in inhouse capsule product disso is paddle with sinker in release media is there then RLD product in tablet form then with same as paddle with sinker we can proceed n.a.?

676

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UV and PDA detector, which have less signal to noise ratio?

1098

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For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?

4030

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why holium oxide prepared in 10% HCLO4 SOLUTION

1421

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what is definition of validation? which components are followed give detail?

2315

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all types of questions asked at the cipla interview

4647

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how to set sample and standard concentration in RS method?

856

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