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Answer Posted / vikram bahadur lal
in spread plate method the mixed culture is not directly
poured into the culture medium,but it is made regularly
diluted into different testtube with the help of sterile
bent glass rod.now a sample is removed from each tube and
placed it into the agar medium.
In pour plate method the mixed culture is diluted directly
,in the cooled agar medium.the medium is maintained in a
cooled liquid state at a temperature of about 45*C.now the
let the medium be cooled so that agar agar medium can easily
get solidify.incubate it overanight .colonies may develop.
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