Why we are maintaining the autoclave temperature at
121degree centigrade and pressure at 15lbs? Why we are not
maintaining the temperature and pressure above or below
121degree centigrade and 15lbs? higher temperature also
kills microbes. then why we are not maintaining it above
121degree? what is the reason?
- 4 Answers
- 37256 Views
- Biocon, I also Faced
- E-Mail Answers
Answer Posted / kk pandey
bcoz the approx 120 degree is the highest temp which the spore
of thermophilic bacteria (Bacillus sterothermophilus)can
withstand.
Hence for autoclaving temp is kept 121 so as to kill even the
spores is killed.
Now why the temp is not increased from 121 is bcoz at higher
temp the media, or other nutritious material which u r
autoclaving can get denatured.
| Is This Answer Correct ? | 43 Yes | 10 No |
Post New Answer View All Answers
How to determine if bacteria is sensitive to an antibiotic?
What are the advantages of quaternary ammonium compounds?
Explain about the reporter gene in lambda phages?
What do you know about our company?
What is meant by biotransformation and swertiamarin?
How to preserve filamentous fungi in distilled water ?
Describe about Proteus Mirabilis rRNA?
How to make antibiotic plates ?
IN autoclave validation, suppose one biological indicator fail among 16 indicator then whether cycle is accepted or not
Why should the slide be flooded with strong carbol fuchsin?
What are the articles sterilized in a hot air oven?
What is meant by fungal element?
Explain about the Resuspending mycobacterial cells?
why microbiology failed to experience a period of growth after the reports of Leeuwenhoek?
Which is the more important step in gram stain?
