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what is the method used to identify your transformed cells
from the plasmid vector?
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Answer Posted / kk pandey
I)If transformant is thought to have antibiotic resistant
gene (called as Marker Gene)along with ur desired gene, then
culture those cells in a media containing that same
antibiotic.
==>Case 1:- the cell is not transformed.:-
the cell will not have the antibiotic resistant gene, hence
it wont grow (or simply die)bcoz of antibiotic in the media.
==>Case 2:-the cell is successfully transformed.:-
The cell will easily grow in media bcoz antibiotic wont
affect it as cell will resist it.(bcoz of marker gene).
-----*----
My answer is quite long but i think i will properly make u
understand.
| Is This Answer Correct ? | 8 Yes | 2 No |
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