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HOw do i calculate the number of theoretical plate on a HPLC
chromatography

Answer Posted / daljit singh randhawa

Theoretical plates(N):-

N = 5.55 x [RT/W]2

Here N = No. of Theoretical plates
RT = Retention Time
W = Peak width at half the hight of peak


Suppose RT of peak is 20 min. & Hight is 10 mm

Then W = 10/2 = 5


so N = 5.55 x [20/5]2

= 5.55 x [4]2
= 5.55 x 16 = 88.80

Is This Answer Correct ?    22 Yes 11 No



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