Interested to Buy Any Domain ? << Click Here >> for more details...
Why it is OD 600nm for optical density investigation ?
- 2 Answers
- 22017 Views
- CDRI, Venus, I also Faced
- E-Mail Answers
Answer Posted / shivesh
When measuring light scattering it is important to consider the wavelength of light used a bacterial culture. Microorganisms may contain numerous macromolecules that will absorb light, including DNA (254 nm), proteins (280 nm), cytochromes (400-500 nm), and possible cell pigments. When measuring bacteria by light scattering it is best to pick a wavelength where absorption is at a minimum and for most bacterial cultures wavelengths around 600 nm are a good choice. However, the exact wavelength chosen is species specific.
This wavelength is not absorbed by much of anything in bacteria so you measure light scattering by the microbes, which is proportional to cell density.
| Is This Answer Correct ? | 35 Yes | 2 No |
Post New Answer View All Answers
How are stains classified?
What is meant by biotransformation and swertiamarin?
what is the method for seperartion of spheroplasts?
Give an account of Viral isolation?
Explain about the reporter gene in lambda phages?
How molecular taxonomy has affected bacterial classification?
How to extract HBV cccDNA from heptoma cell lines?
How to Construct mutant virus with BAC ?
Explain about b-D-glucosidase?
Can reconstituted SMP be autoclaved?
Explain the aminoglycosides resistance problem ?
Explain about the Bacillus pasteurii?
How to grow more methylobacterium?
How to measure or estimate colony form unit(CFU) ?
How to search specific Cell line ?
