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Why it is OD 600nm for optical density investigation ?
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Answer Posted / shivesh
When measuring light scattering it is important to consider the wavelength of light used a bacterial culture. Microorganisms may contain numerous macromolecules that will absorb light, including DNA (254 nm), proteins (280 nm), cytochromes (400-500 nm), and possible cell pigments. When measuring bacteria by light scattering it is best to pick a wavelength where absorption is at a minimum and for most bacterial cultures wavelengths around 600 nm are a good choice. However, the exact wavelength chosen is species specific.
This wavelength is not absorbed by much of anything in bacteria so you measure light scattering by the microbes, which is proportional to cell density.
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