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Answer Posted / tanu talwar
gram staining is a technique to differentiate between gram
positive and gram negative bacterias.
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If a polar molecule has a charge of 4.8 * 10^ (-10) and internuclear distance is 1A then what is its dipole moment?
Explain which branch of science deals with the transformation of chemical energy into electrical energy and vice versa?
What is activation energy?
Give the formula for Peral Ash?
Why are right-handed helices more stable than left-handed helices?
What is the completing sequence of nitrogen-containing bases for an agccgttaac fragment of a dna chain?
Aldehydes and ketones characteristically undergo which reactions?
How are free energy changes related to LeChatelier's Principle?
What does glycolysis in erythrocytes produce?
What is racemic mixture? Why it is optically inactive?
Which organ and subcellular site are most important for fatty acid biosynthesis?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
Explain heat of combustion?
What is meant by substrates of enzymatic reactions?
Explain entropy?
