How you will select the runtime in HPLC for a drug in
method development.
Answer Posted / sarma dvv
In the method development, we have to extend the run time 7-
8 times more with analyte retion time. we have to check any
other peaks are eluting. In that run nopeaks are eluted
means accordingly we can fix the run time.
Generally for isocretic 3times more with resect to analyte
peak.
For Gradient we have to extend the organic concentration
maximum possible.
| Is This Answer Correct ? | 15 Yes | 2 No |
Post New Answer View All Answers
Why are use silicon oil mr?
pharma industries oriented
Explain the relations between number of carbon atoms in alkanes and retention time ?
Why sometime potassium ortho phosphate mix with acetone use for sample preparation for hplc
in dissolution why pool sample needed? in which type of drug pool sample need?
What is third generation HPLC Columns?
If vendor having more imp than monograph then how to proceed? and how to set spec?
what is %labelled amount in content uniformity of dosage unit and its calculation?
analytical method validation require to with respect to release specification or shelf life specification?
For standardization of volumetric solutions, acceptance limit for concentration is considered as 10% of nominal concentration and %RSD as NMT 0.2% (for appropriate no. of replicates). But , if %RSD has not met, what should be further action?
Why we check moisture,ash & AIA in product
could negative ions be produced by bombardment process in mass spectrometry?
Loss of linearity in tkn analysis, possible reasons?
Why dissolution test is not performed in all of the products
Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?