Answer Posted / shilpa tripathi

cupric hydroxide

Define the phenomenon resonance?

1241

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Which are the most abundant metal and third abundant element in the earth crust?

1117

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Explain entropy?

1023

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Define geometrical isomerism.

1168

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give the co-ordination number of cscl?

1252

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Bleaching powder is obtained when cl2 is passes through which inorganic compound?

1092

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What is the super phosphate of lime?

1262

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For what purpose ammonal is used?

1173

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Define polar covalent bond?

1294

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Where is csf found? What is its function?

1130

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Which electrode acts as reference electrode and gives its potential value?

1135

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what are the steps involved in the calibration of HPLC

2740

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Why does physical exertion increase symptoms of poisoning by trematol?

2908

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What is hoffman degradation method?

1140

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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

2301

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