Interested to Buy Any Domain ? << Click Here >> for more details...
what is the general mechanism of high performance liqiud
chromatography?
- 7 Answers
- 18258 Views
- Ajanta Pharma, Amneal Pharmaceuticals, I also Faced
- E-Mail Answers
Answer Posted / nabil
Confusigly,there are two variants in use in HPLC depending
on the relative polarity of the solvent and the stationary
phase.
1-Normal Phase HPLC
The column is with tiny silica particles, and the solvent
is non polar hexane for example, Polar cpds in the mix
being passed through the column will stick longer to the
polar silica than the non polar cpds will, the non polar
ones will therefore pass more quickly through the column.
2-Reversed phase HPLC
In this case the silica is modified to make it non polar by
attacheing hydrocarbon chains to its surface typically with
either 8 or 18 carbon atomsin them. A polar solvent is used.
In this case there will bestrong attraction between polar
solvents and polar molecules will pass through
thecolumntherewon't be much attractoin between the
hydrocarbon chain and the polar solvent.
Non polar cpds in the mix will tend to form attractions
with the hydrocarbons gps because of van der waals forces
they will be also less soluble in the solvent because the
need to break the hydrogen bonds,they spend less time in
solution in the solvent, that mean the polar molecules that
will travel through the column more quickly.
| Is This Answer Correct ? | 4 Yes | 2 No |
Post New Answer View All Answers
what is the origin to prepare standard operating procedure
what is difference in ods I ods II is this is realy from sorbent / packing characteristics or from other means
in dissolution if one bowl got 70 percent 2nd bowl got 80 percent and 3rd bowl got 90 percent then how proceed?
Which products will produce when acetic anhydride reacts with Ter-butanol, Sec-butanol, Iso butanol, Dimethylamine etc.
Hi sir if any product monograph not given known impurities (may be 5 impurities) specifications then how we require to proceed for inhouse formulation?
How to start the dissolution development for unknown tab?
all types of questions asked at the cipla interview
in which situation require to take incident in validation?
how can I make the copper get white bye any salt or acit?
about method developement in hplc
what is biorelivent dissolution media?
when we talk about change in entropy we consider total entropy means of surround +entropy of system but when we talk about change in Gibss free energy we consider entropy of system.explain with reason.
In the HPLC Calibration done wavelength accuracy done between 200nm-280nm .but not done remaining 300-400nm not done ?
What is a difference between potency and purity?
Why a1% value is used for some product ? What is the criteria for selection a1% value ?
