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Answer Posted / venkat.1830@gmail.com
acidic compounds are more retained at low ph ,basic
compounds are more retained at higher ph while neutral
compounds are uneffected. PH can be used as a powerful
selectivity tool for the separation of ionizable
compounds.At PH values used traditionally (PH 4-8 or
depending upon column specifications ),a change in PH can
result in a dramatic shift in retention
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