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Answer Posted / sandeep negi
widal by tube consider to be more accurate than by slide
method
it is considered 2 b more helpful in the diagnosis
as its gives us rough idea about abs present os not and in
how much concentration
widal by tube
> 4 rows r arranged of 8 clean tubes
> 1 2 3 4 5 6 7 8
> 8th row is our negative control
now
1 clean tube for master dilution is taken
this is our master dilution (1:10 )
now transfer this master .5ml to 1st nd 2nd rows
add saline .5ml fron 2nd row to 8th row
u have to tak .5ml saline in 1st row only
here dil will be 1:20 after adding antigen .5ml
from 2nd row .5ml from master +.5 saline form = 1ml which
will be diluted serially
to other tubes horizontally
which will double our dilution afetyr addition of
respectine antigen(0,h,ah nd bh)
(4.5ml salin+.5ml sample)
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