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what are the principles involved in thin layer
chromatography?
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Answer Posted / prashant
it is a physical method of seperation in whichthe
components to be seperated are distributed between 2 phases.
i.e.,1.stationary phase 2.mobile phase.
In tlc, if silica is used as stationary phase then it
principle is partition co-efficient
And if alumina is used then principle is adsorption.
The seperation in TLC involves distributing a mixture of 2
or more substances between a stationary and mobile phase.
seperation depends upon how much they adsorb on the
stationary phase versus how mwch they dissolve in the
mobile phase.
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