Answer Posted / eapen

Column selection depends on
1.HPLC Method adopted whether Normal phase or reverse phase.
2.Number of components present in the sample. if more
components present instead of short columns long columns
must be used.
3.The functional group present in the analyte.
Based on the functional group present we can use either
Amino,Cyano,Carbohydrate etc. columns.
4.Polarity of the components
eg. If a sample contains nonpolar components instead of
C18 column we can use C8 column or C4 columns.

Many times I don't got a caffeine peak in calibration of hplc using guard column ❓

1876

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What is similarity factor and its use in api strength?

1363

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How to set analytical specification for combination products?

873

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Why we use potassium dichromate in uv calibration Exact reason behind it??

1320

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AT WHAT CONDITIONS WILL YOU ANALYSE A SAMPLE,WHICH ARE PRESERVED AT OTHER THAN THE AMBIENT CONDITIONS?(ie COLD STORAGE SAMPLES BELOW 20* CENTIGRADE?

2113

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what is mean by covalidation

830

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how we can identify the impurity is coming below loq at transfering site?

930

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How we performed the force degradation for drug substance, is any specific guideline is available for each parameter(Acidic, basic, oxidation,heat)? what conditions you mentained for above parameters.

2359

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How to set specification of assay, fisdolution and related substances?

931

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How to do regeneration of Metacarb Pb plus column?

2610

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if content uniformity passing but dissolution varrying then what is next step?

936

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Why potassium dichromate is used in calibration of uv?

2038

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why we are using benzene, anyline in acetic anhdride assay titration method?

2169

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1)What's the meaning of Absorption,give a example. 2)What's the meaning of Adsorption,give a example. 2)what is the difference between Absorption and Adsorption.

2938

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how require to select dissolution media? what is discrimination?

838

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