Answer Posted / m

Column selection is the most important part of your HPLC
method development

1 Choose the column according to the your column polarity
and pH range of buffer.

2 Mobile phase composition and what solvent you are used is
also depends

3 Carbon loading and surface area and micron size is also
depends if you are using more buffer concentraion (Aques)
for that hydro columns are also available

over all it depends on your compound and its polarity

Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?

2709

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Difference between hlaf and rlaf

2110

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why multimedia dissolution require to do?

831

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If vendor having more imp than monograph then how to proceed? and how to set spec?

935

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Why potassium dichromate is used in calibration of uv?

1988

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How you develop a method in HPLC?

2617

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How to do regeneration of Metacarb Pb plus column?

2580

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What is Band broading effect explain

1881

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How to calibration of the uv spectroscopy and its test?

1196

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From where require to take the RLD sample?

756

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Why acetonitrile and water are used as extraction solvent when analysis melamine? I thought they are miscible and won't be able to separate...

1691

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In HPLC Calibration, On which basis RSD Limit of noise test is fixed (NMT 33.0 % )

3486

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Tell me about analytical method validation in QC

6181

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on the basis of bcs class how to proceed the solubility?

782

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what is diffrence between specificity and selecivity?

781

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