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Answer Posted / kavitha
IN POur plate technique the sample is serially diluted in
saline(9ml saline+iml sample-1st tube), from the first 1ml
is taken and poured into the 2nd tube.the selected dilution
is taken and from that 1ml is poured into the
petriplate.meanwhile the nutrient agar is prepared and
tempered at 45 degreecelcius,.it is poured on to the sample
and the plate is rotated slightly for uniform spreading of
sample in the agar.then the plates are incubated at 37
degree for 24 hours.subsurface colonies seen.anaerobic
bacteria seen.
in spread plate,it is done in similar way but the 0.1ml
sample is poured on the agar surface.with the l rod it is
evenly spreade and incubate at same temperature.aerobic
bacteria can grow.contamination is possible.both gives
viable count.
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